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Check priceEXTRACTION PROCEDURE 1) Add 250 uL CTAB-BME to the 1.2 mL strip tubes with tissue and grinding ball. 2) Grind at 1700 rpm for 2 minutes. Make sure the boxes are securely fastened in the grinding machine, Double check the buckles and make sure the small metal tabs on the front of the buckle are locked in the down position. This is CRITICAL to
Check priceBionano Prep Plant Tissue DNA Isolation Protocol Liquid nitrogen grinding method for the extraction of DNA from plants. Overview The Bionano Prep Plant Tissue DNA Isolation Protocol Liquid Nitrogen Grinding (LNG protocol) is designed to meet customers needs for high-quality HMW DNA purification from a wide variety of plant species.
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Check pricegel extraction protocol to allow high throughput recovery of DNA samples at a fraction of the cost for ready made kits. The machine-grinding technique is based on the method used by commercially available (e.g. Dyno-Mill, GlenMills,) and previously published (Michaels Amasino 2001) methods, but it reduces equipment cost to a small hand-
Check priceIt is specifically designed for grinding, lysing, and homogenizing biological samples prior to molecular extraction. Wide range of accessories for any sample size 24 x 0.5 mL, 24 x 1.5 mL, 24 x 2 mL, 12 x 7 mL, 3 x 15 mL, 6 x 30 mL, 3 x 50 mL, or 96-well strip well tubes. Ideal homogenizer for extraction of DNA, RNA, proteins, and small
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Check priceDNA Preparation Using Ice-Cap Tissue Samples. As a quantitative reference standard, I used a sample of Arabidopsis genomic DNA that had been purified using a high-yield DNA extraction protocol. The concentration of the DNA from this reference standard was determined using UV spectrophotometry.
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Check priceAfter extraction, an ultra sensitive fluorescent nucleic acid stain, PicoGreen, was used for quantifying trace amount of double-stranded DNA in the solution extracted. For further validation of DNA extraction, four primers were employed to amplify DNA microsatellite loci.
Check priceJun 30, 2014Disruption and Homogenization of Tissue for the Extraction of RNA Tips for optimal tissue disruption and homogenization Use a tube for the homogenization that does not have a conical-shaped bottom. A round or flat bottom tube will allow for a better flow of the material through the probe.
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